|
KMID : 0380220070400050690
|
|
Journal of Biochemistry and Molecular Biology
2007 Volume.40 No. 5 p.690 ~ p.696
|
|
|
Alteration of The Quaternary Structure of Human UDP-Glucose Dehydrogenase by a Double Mutation
|
|
Huh Jae-Wan
Yang Seung-Ju
Hwang Eun-Young
Choi Myung-Min
Lee Hyun-Ju
Kim Eun-A
Choi Soo-Young
Choi Jene
Hong Hea-Nam
Cho Sung-Woo
|
|
|
Abstract
|
|
|
|
There are conflicting views for the polymerization process of human UDP-glucose dehydrogenase (UGDH) and no clear evidence has been reported yet. Based on crystal coordinates for Streptococcus pyogenes UGDH, we made double mutant A222Q/S233G. The double mutagenesis had no effects on expression, stability, and secondary structure. Interestingly, A222Q/S233G was a dimeric form and showed an UGDH activity, although it showed increased Km values for substrates. These results suggest that Ala222 and Ser233 play an important role in maintaining the hexameric structure and the reduced binding affinities for substrates are attributable to its altered subunit communication although quaternary structure may not be critical for catalysis.
|
|
|
KEYWORD
|
|
|
Cassette mutagenesis, Gel filtration, Polymerization, UDP-glucose dehydrogenase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|